ASPM-associated stem cell proliferation is involved in malignant progression of gliomas and constitutes an attractive therapeutic target

doi:10.1186/1475-2867-10-1

Cancer Cell International

Volume 10

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Bikeye SNN
Colin C
Marie Y
Vampouille R
Ravassard P
Rousseau A
Boisselier B
Idbaih A
Calvo CF
Leuraud P
Lassalle M
El Hallani S
Delattre JY
Sanson M

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Bikeye SNN
Colin C
Marie Y
Vampouille R
Ravassard P
Rousseau A
Boisselier B
Idbaih A
Calvo CF
Leuraud P
Lassalle M
El Hallani S
Delattre JY
Sanson M
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ASPM-associated stem cell proliferation is involved in malignant progression of gliomas and constitutes an attractive therapeutic target

Sandra-Nadia Ngwabyt Bikeye¹ , Carole Colin² , Yannick Marie¹ , Raphaël Vampouille⁴ , Philippe Ravassard⁴ , Audrey Rousseau⁵ , Blandine Boisselier¹ , Ahmed Idbaih¹^,3 , Charles Félix Calvo¹ , Pascal Leuraud⁶ , Myriam Lassalle⁶ , Soufiane El Hallani¹ , Jean-Yves Delattre¹^,3 and Marc Sanson¹^,3

¹UMR975 INSERM-UPMC, GH Pitié- Salpêtrière, Paris

²UMR911-CRO2, Faculté de Médecine Timone, Université de la Méditerranée, Marseille

³Service de Neurologie Mazarin, GH Pitié- Salpêtrière, APHP, Paris

⁴Biotechnology & Biotherapy laboratory, Centre de Recherche de l'Institut du Cerveau et de la Moelle, CNRS UMR9225, INSERM UMRS 975 Université Pierre et Marie Curie, Hôpital de la Salpêtrière, Paris

⁵Service de Neuropathologie Raymond Escourolle, GH Pitié- Salpêtrière, Paris

⁶XenTech, Evry, France

author email corresponding author email

Cancer Cell International 2010, 10:1doi:10.1186/1475-2867-10-1


Published:	11 January 2010

Additional files

Additional file 1:

Figure S1 - ASPM protein expression. ASPM western blot analysis of grade II, III and IV gliomas (n = 4 in each group). Relative protein expression is presented as the ratio of density value for ASPM over GAPDH signal (mean +/- SEM).

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Additional file 2:

Figure S2 - Tumor spheroid characterization. Genomic stability was examined with CGHa analysis (left = DNA profile from initial tumor; right = DNA profile from gliomasphere at passage p28). The chromosomes are indicated on the x axis and copy number is on y axis. Yellow indicates the normal genomic copy number, while green indicates a loss and red indicates a gain in copy number. As shown here, GBM1 carries EGFR amplification and loss of chromosome 10q. After CSC isolation in stem cell-permissive medium, gliomaspheres were differentiated in presence of fetal bovine serum and stained by Sox2 and O4 for stemness markers and by GFAP and Tuji1 to characterize the potential of multi lineage differentiation of GBM1.

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Additional file 3:

Figure S3 - Knock-down of Aspm gene. Tumor spheroids (GBM1, GBM2, GBM3) were transfected either with non silencing (scrambled ShRNA) or with ASPM ShRNA expressing lentivirus, resulting in a dramatic drop of ASPM RNA in GBM1, GBM3 and to a lesser extent GBM2.

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