Figure 1.

Panel A. Levels of γ-interferon mRNA following the administration of GM-CSF after chemotherapy. γ-interferon mRNA levels were measured in 16 patients pre-chemotherapy/GM-CSF administration and post-GM-CSF/chemotherapy administration by quantitative real time PCR of peripheral blood mononuclear cells. γ-interferon mRNA levels were normalized against the housekeeping gene β-actin mRNA. There was no statistically significant change between the two groups. Panel B. Levels of T-bet transcription factor mRNA following the administration of GM-CSF after chemotherapy. T-bet transcription factor mRNA levels were measured in 16 patients pre-chemotherapy/GM-CSF administration and post-GM-CSF/chemotherapy administration by quantitative real time PCR of peripheral blood mononuclear cells. T-bet transcription factor mRNA levels were normalized against the housekeeping gene β-actin mRNA. There was an approximate 1.7 fold decrease in T-bet transcription factor mRNA levels following the administration of GM-CSF after chemotherapy (p = 0.043). The statistical significance was determined by a Wilcoxin matched pairs test.