Investigation of the apoptotic way induced by digallic acid in human lymphoblastoid TK6 cells
1 Laboratoire de biologie Cellulaire et Moléculaire, Faculté de Medecine Dentaire Monastir, Rue Avicenne, Monastir, 5000 Tunisia
2 Unité de pharmacognosie 99/UR/07, Faculté de pharmacie Monastir, Rue Avicenne, Monastir, 5000 Tunisia
3 Faculty of Dental Medicine, Rue Avicenne, Monastir, 5019, Tunisia
Cancer Cell International 2012, 12:26 doi:10.1186/1475-2867-12-26Published: 11 June 2012
The digallic acid (DGA) purified from Pistacia lentiscus. L fruits was investigated for its antiproliferative and apoptotic activities on human lymphoblastoid TK6 cells.
We attempt to characterize the apoptotic pathway activated by DGA. Apoptosis was detected by DNA fragmentation, PARP cleavage and by evaluating caspase activities.
The inhibition of lymphoblastoid cell proliferation was noted from 8.5 μg/ml of DGA. The induction of apoptosis was confirmed by DNA fragmentation and PARP cleavage. We have demonstrated that DGA induces apoptosis by activating the caspase-8 extrinsic pathway. Caspase-3 was also activated in a dose dependent manner.
In summary, DGA exhibited an apoptosis inductor effect in TK6 cells revealing thus its potential as a cancer-preventive agent.