Typical TIC of the VOCs from the urine samples obtained from the tumor-bearing (A) and normal control mice (B). The TIC was obtained from the analysis of urine samples (200 μL) by HS-SPME (DVB/CAR/PDMS, 50/30 μm, 2 cm) and GC-TOF MS. The extraction temperature was 45°C and the time was 50 min. Desorption was performed at 240°C for 10 min. The injection was pulsed splitless (closed 3 min) with a 0.75 mm liner. Temperature programming was set at an initial temperature of 40°C for 5 min, then programmed at 3°C/min to 240°C with a 5 min hold at this final temperature. All other GC-MS conditions are described in the Material and Methods.
Hanai et al. Cancer Cell International 2012 12:7 doi:10.1186/1475-2867-12-7