Effect of the inhibition of RAIDD expression on the growth and survival of SK-BR-3 and MCF-7 cells after paclitaxel treatment. (A) Efficiency of RAIDD suppression by two employed specific siRNA, i.e. A and B, in SK-BR-3 and MCF-7 cells is shown. Levels of RAIDD were determined using western blot analysis and relevant antibodies (see “Materials and Methods”). Actin levels were used to confirm equal protein loading. (B) The effect of specific RAIDD siRNA on the growth and survival of SK-BR-3 and MCF-7 cells after paclitaxel (T) treatment (100 nM for SK-BR-3 cells and 300 nM for MCF-7 cells) is presented. The cells were seeded at 7 × 103 cells/200 μl of medium per well and prepared as described (see “Materials and Methods”). After 0, 48 and 96 h of incubation, the number of living cells was determined (see “Materials and Methods”). Each column represents the mean of 2 separate cultures ± SEM.
Jelínek et al. Cancer Cell International 2013 13:42 doi:10.1186/1475-2867-13-42