Effect of paclitaxel and SB-T-1216 on (A) the level of procaspase-2, cleaved caspase-2, and (B) the level of golgin-160 in SK-BR-3 and MCF-7 cells. After 0, 12, 24, 36, 48 and 60 h of incubation with paclitaxel or SB-T-1216 (100 nM for SK-BR-3 cells and 300 nM for MCF-7 cells), levels of procaspase-2, cleaved caspase-2 and levels of golgin-160 were determined using western blot analysis and relevant antibodies (see “Materials and Methods”). Actin levels were used to confirm equal protein loading. The data shown were obtained in one representative experiment of three (procaspase-2, cleaved caspase-2) or two (golgin 160) independent experiments. MCF-7 data demonstrating the level of procaspase-2 and the level of cleaved caspase-2 are from different experiments.
Jelínek et al. Cancer Cell International 2013 13:42 doi:10.1186/1475-2867-13-42