Effect of paclitaxel on coimmunoprecipitation of caspase-2 and PIDD with RAIDD in SK-BR-3 and MCF-7 cells. After 24 h of incubation with paclitaxel (100 nM for SK-BR-3 cells and 300 nM for MCF-7 cells), the level of precipitated RAIDD and coimmunoprecipitated caspase-2 as well as PIDD (detected by antibody against C-form) were determined using western blot analysis and relevant antibodies (see “Materials and Methods”). The heavy chain of the antibody used for RAIDD precipitation (Ab) is seen. The data shown were obtained in two independent experiments.
Jelínek et al. Cancer Cell International 2013 13:42 doi:10.1186/1475-2867-13-42