Table 4

Results of quantitative RT-PCR for antioxidant enzyme gene expression in the heart of mice fed the AIN·76 food formula with (+) or without (-) a synthetic vitamin E supplement (2000 IU/kg dry food) for six weeks.


Enzymea
Vitamin E supplement
 GPX-1
 CAT
 CZ SOD
 Mn SOD
 EC SOD
 HO-1b
 Mn SODb GPX-1
-
 .325 ± .019
 .495 ± .048
 .915 ± .038
 2.106 ± .166
 .064 ± .005
 .020 ± .002
 6.55 ± 0.56
+
 .252 ± .028
 .522 ± .029
 .915 ± .054
 2.538 ± .175
 .059 ± .005
 .013 ± .002
 10.49 ± 1.16

a Abbreviations: GPX-1 – glutathione peroxidase; CZ SOD – copper zinc superoxide dismutase; CAT – catalase; Mn SOD – manganese superoxide dismutase; EC SOD – extracellular superoxide dismutase; HO-1-inducible form of heme oxygenase b Means differ to significant extent using two-tailed t test. Data are presented as the mean ± SE number of transcripts for each mRNA after normalization to expression of beta actin. N = 5 mice per group.

Cameron et al. Cancer Cell International 2003 3:3   doi:10.1186/1475-2867-3-3