Table 2

Effect of vanadium and beta-Carotene on the number of single-strand breaks/DNA fragment in rat liver 18–20 h after a single injection of DEN

Group
Treatment
Percentage of Double-strand DNA
Number of single-strand breaks/DNA fragment
Inhibition (%)

A
Normal
93.56
0.07 ± 0.02a

B
DENA Control
31.68
1.32 ± 0.01 b

C
DENA+V
47.23
0.58 ± 0.01 c
56.06
D
DENA+BC
52.87
0.54 ± 0.04 c
59.09
E
DENA+V+BC
64.73
0.48 ± 0.023 d
63.6

The mean values of the number of the single-strand breaks/DNA fragment for Vanadium control (group c) was 0.07 ± 0.01; for β-carotene control (group d), 0.08 ± 0.01 and the Vanadium and beta-carotene control (group e), 0.10 ± 0.01. No statistical significance could be observed between these drug controls when compared with normal vehicle control (Group A).

aValues are means ± S.E of 5 rats (each rat liver genomic was tested in triplicate)

bp < 0.01 as compared with group A,

cp < 0.01 as compared with group B,

dp < 0.005 as compared with group B.

Chattopadhyay et al. Cancer Cell International 2005 5:16   doi:10.1186/1475-2867-5-16