Table 3

NMR peak ratios measured on K562wt and K562adr spectra. PBS: incubation in PBS; PFA: incubation in PFA; Triton : incubation in PFA and triton X; Smase: incubation in PFA, triton X-100 and 0.5 units sphingomyelinase. Results are expressed as means of arbitrary units ± SD multiplied by ten.

K562wt
 K562adr
Group
 PBS (n = 4)
 PFA (n = 7)
 Triton (n = 7)
 Smase (n = 4)
 PBS (n = 4)
 PFA (n = 7)
 Triton (n = 8)
 Smase (n = 5)
CH3/Ct
 198 ± 21
 231 ± 12
 312 ± 42*
 392 ± 105°
 161 ± 19
 192 ± 22
 265 ± 62*
 267 ± 42*
CH2/Ct
 541 ± 52
 611 ± 78
 1060 ± 164*
 1696 ± 441°
 308 ± 93
 386 ± 56
 695 ± 141*
 565 ± 167*
N(CH3)3/Ct
 132 ± 6
 141 ± 18
 53 ± 10*
 79 ± 17°
 114 ± 12
 132 ± 36
 60 ± 15*
 94 ± 39*

Statistical analysis : * : P < 0.05 when compared with PBS-incubated cells,

°: P < 0.05 when compared with Triton-treated cells,

Mannechez et al. Cancer Cell International 2005 5:2   doi:10.1186/1475-2867-5-2