Table 1

Amino acid sequences of Pep27 derived from S. pneumoniae and its analogue peptides, and their retention times

Peptide
Amino acid sequences
Remarks (substitution)
RT (min)

Pep27
MRKEFHNVLSSGQLLADKRPARDYNRK
(2R→W), (4E→W)
14.54
Pep27anal1
MWKWFHNVLSSWQLLADKRPARDYNRK
(2R→W), (4E→W)
18.67
Pep27anal2
MWKWFHNVLSWWWLLADKRPARDYNRK
(2R→W), (4E→W), (11S→W), (13Q→W)
22.50
Pep27anal3
MRKWFHNVLSSGQLLADKWPAWDYNRK
(4E→W), (19R→W), (22R→W), (26R→W)
20.08
Pep27anal4
MWKEFHNVLSSGQLLADKRWARWYNRW
(2R→W), (20P→W), (23D→W), (27K→W)
19.15
Pep27anal5
MWKWFHNVLSSGQLLADKWWAWWYNWW
(2R→W), (4E→W), (19R→W), (20P→W), (22R→W), (23D→W), (26R→W), (27K→W)
19.17

RT, retention time obtained by RP-HPLC

Lee et al. Cancer Cell International 2005 5:21   doi:10.1186/1475-2867-5-21