A new, fast and semi-automated size determination method (SASDM) for studying multicellular tumor spheroids

doi:10.1186/1475-2867-5-32

Cancer Cell International

Volume 5

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Monazzam A
Razifar P
Lindhe Ö
Josephsson R
Långström B
Bergström M

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Razifar P
Lindhe Ö
Josephsson R
Långström B
Bergström M
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Primary research

A new, fast and semi-automated size determination method (SASDM) for studying multicellular tumor spheroids

Azita Monazzam¹^,3 , Pasha Razifar²^,3 , Örjan Lindhe³ , Raymond Josephsson⁵ , Bengt Långström³ and Mats Bergström³^,4

¹Institute of Oncology, Radiology and Clinical Immunology, Uppsala University

²Uppsala University, Centre for Image Analysis, Lägerhyddsvägen 3, SE-752 37 Uppsala, Sweden

³Uppsala Imanet AB (PET Centre), Uppsala University Hospital, SE-751 85 Uppsala, Sweden

⁴Department of Pharmaceutical Biosciences, Uppsala Biomedical Centre, SE-751 24 Uppsala, Sweden

⁵Department of medical Science, Uppsala University Hospital, SE-751 58 Uppsala, Sweden

author email corresponding author email

Cancer Cell International 2005, 5:32doi:10.1186/1475-2867-5-32


Published:	14 November 2005

Abstract

Background

Considering the width and importance of using Multicellular Tumor Spheroids (MTS) in oncology research, size determination of MTSs by an accurate and fast method is essential. In the present study an effective, fast and semi-automated method, SASDM, was developed to determinate the size of MTSs. The method was applied and tested in MTSs of three different cell-lines. Frozen section autoradiography and Hemotoxylin Eosin (H&E) staining was used for further confirmation.

Results

SASDM was shown to be effective, user-friendly, and time efficient, and to be more precise than the traditional methods and it was applicable for MTSs of different cell-lines. Furthermore, the results of image analysis showed high correspondence to the results of autoradiography and staining.

Conclusion

The combination of assessment of metabolic condition and image analysis in MTSs provides a good model to evaluate the effect of various anti-cancer treatments.