Figure 5.

NFAT-DNA-binding activity in normal and cervical cancer nuclear extracts. Briefly 100 μg of nuclear extracts was incubated with ³²P-labelled NFATc oligonucleotide and Electrophoretic mobility shift assay was performed as described in Material and methods. Lane one contains free probe without nuclear extract and lane 2 contains 100-fold excess of unlabelleled NFAT oligonucleotide as a specific competitor. Specific NFAT- DNA complexes are indicated by arrow.