Figure 4.

Light microscopy photos of the mutant p53 cell line MEF 10(1) following 10 Gy γ-irradiation. Methanol/glacial acetic acid fixed cells were stained using Hoechst 33258 and visualized using fluorescence microscopy, as described in Materials and Methods. Panel A: morphology of MEF 10(1) cells at 32 hours post-irradiation. Panel B: morphology of sham irradiated MEF 10(1) cells, at time zero.