Figure 7.

Expression of cyclin B1 in the mutant p53 cell line MEF 10(1) and in the wild-type p53 cell line MEF 12(1) following 10 Gy γ-irradiation. Thirty μg total proteins from whole cell extracts were resolved using SDS-PAGE, and processed as described in Materials and Methods. Immunoblots were visualized using chemiluminesence detection. Lanes 1–5: p53 mutant MEF 10(1) cells: sham-irradiated control at zero time (line 1), followed by 0, 8, 24, and 32 hours post-irradiation samples. Lanes 6–10: wild-type p53 MEF 12(1) cells: sham-irradiated control at zero time (line 6), followed by 0, 8, 24, and 32 hours post-irradiation samples.