Figure 3.

Flow cytometry of uPAR cell surface expression shows development of a high uPAR population during suspension culture. A. Representative flow cytometry histograms of uPAR expression on PC-3M cells over a 5 day time course in forced suspension culture. M1 marker shows region greater than background fluorescence (secondary antibody-only control). B. Bars represent the mean fluorescent intensity measured in fluorescent counts of uPAR expression in un-fixed PC-3M cells. Cells were incubated in suspension as described in methods for the time periods shown. Bars are the mean of 4 replicates ± s.d.