Table 3

Quantitative characteristics of the labeled material content in various cell compartments depending on the time of its incubation with cells

Incubation time

0 min

15 min

30 min

60 min

120 min

180 min

Experiment no.

II

III

IV

II

III

IV

II

III

IV

II

III

IV

II

III

IV

II

III

IV

Cytoplasmic fraction (absolute counts, cpm)

74 883

470 559

275 481

542 304

674 216

677 588

980 421

854 765

960 000

1 060 674

518 282

863 345

368 101

% of the amount added to medium

0.7%

2.3%

1.5%

5.0%

6.2%

6.3%

4.8%

4.7%

4.7%

5.8%

4.8%

4.2%

2.0%

Amount in μg according to %

0.0098

0.08

0.0375

0.07

0.087

0.088

0.17

0.118

0.17

0.145

0.67

0.15

0.05

Interchromosomal fraction (absolute counts, cpm)

11 460

3060

7330

83 662

95 821

97 147

159 378

322 008

299 963

445 426

177 814

282 826

203 421

% of the amount added to medium

0.1%

0.015%

0.04%

0.77%

0.89%

0.9%

0.78%

1.75%

1.47%

2.42%

1.65%

1.39%

1.1%

Amount in μg according to %

0.0014

0.0005

0.0025

0.01

0.013

0.0135

0.03

0.04

0.053

0.061

0.023

0.05

0.028

% of the genome

0.066%

0.016%

0.083%

0.33%

0.43%

0.45%

1.0%

1.33%

1.77%

2.03%

0.73%

1.67%

0.92%

Chromatin (absolute counts, cpm)

8089

3691

2248

45 812

47 684

49 394

296 771

123 390

242 836

157 124

37 620

244 937

118 285

% of the amount added to medium

0.075%

0.018%

0.012%

0.4%

0.44%

0.46%

1.45%

0.67%

1.19%

0.85%

0.35%

1.2%

0.64%

Amount in μg according to %

0.0011

0.00065

0.0003

0.0056

0.006

0.0064

0.05

0.0168

0.0428

0.021

0.0042

0.043

0.016

Rogachev et al. Cancer Cell International 2006 6:23   doi:10.1186/1475-2867-6-23