Figure 1.

Morphological changes of the HCOL1A1-overexpressing T98G cells. Wild cells (Wild), control transfected cells (Mock), and HCOL1A1-transfected cells (HCOL1A1-I and HCOL1-II) were seeded at 1 × 10⁵ cells on 6-cm culture dishes and grown for 2 to 4 days. (a) Immunocytochemical staining of T98G cells (day 2). HCOL1A1 peptides were immunostained with polyclonal anti-α1(I) collagen antibody. Scale bar: 100 μm. (b) Phase-contrast photomicrographs of T98G cells (day 4). Scale bar: 200 μm.