Figure 8.

The percentage cleavage of oligonucleotide substrate containing DHU lesion. The radiolabeled oligonucleotide substrate was incubated with cell extracts prepared from either unirradiated or 3 Gy irradiated cells collected at various time points. The reaction products were separated on polyacrylamide gel and the incised fragments were quantified to calculate the percentage cleavage at the DHU site.