Table 1

Differentially expressed genes after UVA dose of 8 J/cm2
Gene family [Swiss prot Accession #]
 No. of arraysa
 Controlb ± SD
 UVA exposedc ± SD
 t-test
 Ratiod
 Function
Stress induced
Heat shock 86-kDa protein (HSP86) [P07901]
 3 ↑
 0,32 ± 0,23
 1,04 ± 0,44
 0,43
 3,23
 Belongs to HSP90 family; cytoplasmic molecular chaperone regulating the correct folding in the heat induced conformational changes.
Heat shock cognate 71-kDa (HSC70; HSP73), mouse homolog of human [P11142]
 2 ↑
1 ↓
 0,15 ± 0,12
 0,27 ± 0,18
 0,39
 1,81
 Belongs to HSP70 family; molecular chaperone regulating the correct folding; found in melanoma cell lines [33].
Alpha crystallin B-subunit, mouse homolog of human [P02511]
 3 ↑
 0,07 ± 0,03
 0,21± 0,07
 0,05
 3,26
 Belongs to HSP20 family; found in mammalian transparent lens in eye induced by stress [34,35].
Oxidative Stress
Oxidative stress-induced protein (OSI) [Q64337]
 2 ↑
1 ↓
 0,16 ± 0,14
 0,31± 0,15
 0,27
 1,92
 Regulates metabolic responses to oxidative stress, were also induced [36]
glutathione S-transferase mu2 (GSTM2); [P15626]
 3 ↑
1 ↓
 0,07 ± 0,04
 0,14 ± 0,09
 0,23
 2,09
 Belongs to the GST superfamily; prevents the toxic injuries; expressed in human melanoma cells [37] and in keratinocytes from human squamous cell carcinoma [38].
Cell cycle control
cyclin G [O54779; P51945]
 5 ↑
 0,13 ± 0,04
 0,65 ± 0,36
 0,03
 4,85
 Contributes to G2/M arrest in response to DNA damage; a transcriptional target of the p53.
Angiogenesis
Vascular endothelial growth factor (VEGF) [Q00731]
 4 ↑
 0,07 ± 0,03
 0,22 ± 0,08
 0,03
 2,94
 Promotes endothelial cell proliferation and migration in angiogenesis; permeabilizes the blood vessels; expression regulated by UVB in keratinocytes and fibroblasts [39,40].
Skeleton & Motility protein
G-actin cytoplasmic [P02571; P14104]
 3 ↓
 1,02 ± 0,37
 0,47 ± 0,18
 0,10
 0,47
 Exists in all eukaryotic cells as a component of the cytoskeleton mediating cell motility. Polymerization leads to formation of filamentous F-actin.
non-muscle cofilin 1 (CFL1) [P18760]
 3 ↓
 0,40 ± 0,10
 0,17 ± 0,01
 0,05
 0,41
 Controls reversibly actin depolymerization from F-actin to G-actin causing an increase in the G-actin pool.

a The number of arrays (out of five), in which differences in the gene expression were observed.; The arrows indicate the upregulation or downregulation of the gene. In the remaining arrays no difference was observed.

b The average of the normalized gene intensity in control cells ± standard deviation;

c The average of the normalized gene intensity in UVA exposed cells ± standard deviation.

d Ratio of UVA exposed genes versus control genes.

Pastila and Leszczynski Cancer Cell International 2007 7:7   doi:10.1186/1475-2867-7-7