24-bit fluorescence (A) and 8-bit gray-tone (B) images of small neoplastic lymphocyte. Subtracted 55 to remove background pixels; added 36 to set highest reference pixel point to 100 as shown by the point of arrow in panel B corresponding to the value 100 in the adjoining Table. The curve has a narrower peak, and 9 spikes mostly at the sides of the peak. It also has a left shoulder with minute spikes.
Isitor et al. Cancer Cell International 2009 9:13 doi:10.1186/1475-2867-9-13