Primary research

Growth inhibition of HeLa cell by internalization of Mycobacterium bovis Bacillus Calmette-Guérin (BCG) Tokyo

Akira Kitamura¹ , Sohkichi Mastumoto² and Izumi Asahina¹

¹  Division of Oral and Maxillofacial Surgical Reconstruction and Functional Restoration, Department of Developmental and Reconstructive Medicine, Graduate School of Biomedical Sciences, Nagasaki University, Sakamoto, Nagasaki 852-8588, Japan

²  Department of Host Defense, Osaka City University. Graduate School of Medicine, Asahi-machi, Abeno-ku, Osaka 545-8585, Japan

author email corresponding author email

Cancer Cell International 2009, 9:30doi:10.1186/1475-2867-9-30

Published:	2 December 2009

Abstract

Background

Intravesical BCG immunotherapy is effective for preventing recurrence and progression in none muscle-invasive bladder cancer but the dosing schedule and duration of treatment remain empirical. The mechanisms by which intravesical BCG treatment mediates antitumor activity are currently poorly understood.

Results

HeLa cell infected with Mycobacterium bovis Bacillus Calmette-Guérin(BCG) Tokyo which were different multiplicity of infection(MOI). Proliferation of HeLa cell reduced in a dose-dependent manner by live BCG. The cytoplasm of the HeLa cell showed variety lysosomal stages by internalized and interacted BCG.

Conclusion

Proliferated Live BCG secreted the protein and depressed the growth of tumor. The possibility for clinical introduction of BCG therapy for carcinoma reported with review of literature.